Reprinted with permission from ACS Appl. Mater. Interfaces 2020, 12, 8, 9977-9988. Copyright 2020 American Chemical Society.
ACS Appl. Mater. Interfaces, 12, 8, 9977-9988 (2020)
Monoclonal antibodies have become leading candidates to be used as therapeutics due to the exceptional selectivity and binding affinity for their molecular targets. However, the absorption of antibodies at air-water interfaces often occurs during manufacturing, transportation and even delivering therapeutic drugs to the patients. And the subsequent denaturation after the absorption reduces their lives. To overcome this problem, the pharmaceutical industry uses surfactants to compete with antibodies for adsorption onto the surface. A research group from CCNY used pendant bubble tensiometry and X-ray reflectivity techniques to characterize the adsorption process of antibodies and surfactants after they are premixed in the water.
Ankit D. Kanthe 1, Mary Krause 2, Songyan Zhen 2, Andrew Ilottv 2, Jinjiang Li 2, Wei Bu 3, Mrinal K. Bera 3, Binhua Lin 3, Charles Maldarelli* 1,4, and Raymond S. Tu*1
1 Department of Chemical Engineering, The City College of New York, New York, NY 10031, USA.
2 Drug Product Science and Technology, Bristol-Myers Squibb, New Brunswick, NJ 08901, USA.
3 NSF’s ChemMatCARS, Center for Advanced Radiation Sources, c/o Advanced Photon Source/ANL, The University of Chicago, Argonne, IL 60439, USA.
4 Levich Institute, The City College of New York, New York, NY 10031